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TMB was calculated because the ratio between the variety of mutations and the size of the sequenced regions (35.7 Mb). For these patients with available RNAseq, quartiles of FPKM have been calculated. Mutations identified by WES analysis were considered as “expressed mutations” if no less than one of many gene counter algorithms was expressed in third or 4th quartile. Once mutations have been filtered, 29-mer amino acid sequences, containing the mutated residue at place 15, were designed.

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The high quality is not nice but we decided to publish it anyway. MC703 cells had been transduced twice with retrovirus-containing supernatants in the presence of polybrene (10 μg/mL) . Transduced cells have been used to gauge the T cell response as described below. RNA was purified from tumor biopsy and preserved at -80 °C.

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